Physical exercise protects dynamic balance and motor coordination of rats treated with vincristine

Luiza Minato Sagrillo, Viviane Nogueira de Zorzi, Luiz Fernando Freire Royes, Michele Rechia Fighera, Beatriz da Silva Rosa Bonadiman, Maria Fernanda Manica Rizzi Cattani, Aron Ferreira da Silveira


Physical exercise has been shown to be an important modulator of the antioxidant system and neuroprotective in several diseases and treatments that affect the central nervous system. In this sense, the present study aimed to evaluate the effect of physical exercise in dynamic balance, motor coordination, exploratory locomotor activity and in the oxidative and immunological balance of rats treated with vincristine (VCR). For that, 40 adult rats were divided into two groups: exercise group (6 weeks of swimming, 1h/day, 5 days/week, with overload of 5% of body weight) and sedentary group. After training, rats were treated with 0.5 mg/kg of vincristine sulfate for two weeks or with the same dose of 0.9% NaCl. The behavioral tests were conducted 1 and 7 days after each dose of VCR. On day 15 we carried out the biochemical analyzes of the cerebellum. The physical exercise was able to protect against the loss of dynamic balance and motor coordination and, had effect per se in the exploratory locomotor activity, and neutralize oxidative stress, damage DNA and immune damage caused by VCR up to 15 days after the end of the training protocol. In conclusion, we observed that previous physical training protects of the damage motor induced by vincristine.

Key-words: exercise, oxidative stress, neuroprotection, cerebellum.

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Bray F, Jemal A, Grey N, Ferlay J, Forman D. Global cancer transitions according to the Human Development Index (2008–2030): a population-based study. The Lancet Oncology 2012;13(8):790-801.

Torre La, Bray F, Siegel EL, Ferlay J, Lortet-Tieulent J, Hemal A. Global cancer statistics, 2012. CA J Cancer Clin 2015;65(2):87-108.

Fukumasu H. Sobre os efeitos quimiopreventivos e antitumorais do guaraná, Paullinia cupana Mart var. sorbilis, em modelos experimentais in vivo e in vitro [Tese]. São Paulo: Universidade de São Paulo; 2008.

Mora E, Smith LML, Donohoe C, Hertz DL. Vincristine-induced peripheral neuropathy in pediatric cancer patients. Am J Cancer Res 2016;6(11):2016.

ANVISA - Agência Nacional de Vigilância Sanitária. (2008). [citado 2016 Abril 12]. Disponível em:[25283-1-0].PDF

Wick A, Wick W, Hirrlinger J, Gerhardt E, Drigen R, Dichgans J et al. Chemotherapy‐induced cell death in primary cerebellar granule neurons but not in astrocytes: in vitro paradigm of differential neurotoxicity. J Neurochem 2004;91(5):1067-74.

Martins DB, Lopes STA, Mazzanti C M, Spanevello R, Schmatz R, Corrêa M et al. Peroxidação lipídica em ratos tratados com sulfato de Vincristina e decanoato de nandrolona. Arq Bras Med Vet Zootec 2011;63(1):107-13.

Warburton DE, Nicol CW, Bredin SS. Health benefits of physical activity: the evidence. CMAJ 2006;174(6):801-9.

Etnier JL, Salazar W, Landers DM, Petruzzello SJ, Han M, Nowell P. The influence of physical fitness and exercise upon cognitive functioning: A meta-analysis. Journal of Sport and Exercise Psychology 1997;19(3):249-77.

Suzuki K. Chronic inflammation as an immunological abnormality and effectiveness of exercise. Biomolecules 2019;7;9(6):223.

Rambo LM, Ribeiro RL, Oliveira MS, Furian AF, Lima FS, Souza MA et al. Additive anticonvulsant effects of creatine supplementation and physical exercise against pentylenetetrazol-induced seizures. Neurochem Int 2009;55(5):333-40.

Steiner SR, Philbert MA. Proteomic identification of carbonylated proteins in 1, 3-dinitrobenzene neurotoxicity. Neurotoxicology 2011;32(4):362-73.

Lee JM, Shin MS, Ji ES, Kim TW, Cho HS, KimCJ et al. Treadmill exercise improves motor coordination through ameliorating Purkinje cell loss in amyloid beta23-35-induced Alzheimer’s disease rats. J Exerc Rehabil 2014;10(5):258.

Ozbeyli D, Gokalp AG, Koral T, Ocal OY, Dogan B, Akakin D et al. Protective effect of exercise and sildenafil on acute stress and cognitive function. Physiol Behav 2015;1(151):230-7.

Gobatto CA, Mello MA, Sibuya CY, Azevedo JR, Santos LA, Kokubun E. Maximal lactate steady state in rats submitted to swimming exercise. Comp Biochem Physiol A Mol Integr Physiol 2001;130(1):21-7.

Souza MA, Oliveira MS, Furian AF, Rambo LM, Ribeiro LR, Lima FD et al. Swimming training prevents pentylenetetrazol‐induced inhibition of Na+, K+‐ATPase activity, seizures, and oxidative stress. Epilepsia 2009;50(4):811-23.

Nadulska A, Klukowska L, Dyba S. Changes of resistance parameters of femoral bone in adult female rats after application of zoladex and vincristin. In: Annales Universitatis Mariae Curie-Sklodowska. Sectio D: Medicina, 2002. p.426-430.

Shabani M, Larizadeh MH, Parsania S, Shekaari MA, Shahrokhi N. Profound destructive effects of adolescent exposure to vincristine accompanied with some sex differences in motor and memory performance. Can J Physiol Pharmacol 2012;90(4):379-86.

Ali S, Lebel C, Bonu S. Reactive oxygen species formation as a biomarker of methylmercury and trimethyltin neurotoxicity. Neurotoxicology 1992;13(3):637-48.

Ha TTN, Huy NT, Murao LA, Lan NTP, Thuy TT, Tuan HM, et al. Elevated levels of cell-free circulating DNA in patients with acute dengue virus infection. PloS one 2011;6(10):25969.

Aebi H. Catalase in vitro. Methods Enzymol 1984;105:121-6.

Misra HP, Fridovich I. The generation of superoxide radical during the autoxidation of hemoglobin. J Biol Chem 1972;247(21):6960-2.

Van den Berg HW, Desai ZR, Wilson R, Kennedy G, Bridges JM, Shanks RG. The pharmacokinetics of vincristine in man. Cancer chemotherapy and pharmacology 1982;8(2):215-9.

Sylvia FLSBV, De Oliveira B, Da Silva Hucke ÉET. A ação do decanoato de nandrolona (Deca-durabolin®) sobre parâmetros hematológicos e proteína total plasmática de ratos (Rattus rattus) com depressão medular induzida após administração de sulfato de vincristina (Oncovin®). Ciência Rural 2005;35(3).

Barzegar-Fallah A, Alimoradi H, Mehrzadi S, Barzegar-Fallah N, Zendedel A, Abbasi A, Dehpour AR. The neuroprotective effect of tropisetron on vincristine-induced neurotoxicity. Neurotoxicology 2014;41(1):1-8.

Quasthoff S, Hartung HP. Chemotherapy-induced peripheral neuropathy. J Neurol 2002;249(1):9-17.

Coufal N, Farnaes L. The Vinca Alkaloids. In: Cancer management in man: chemotherapy, biological therapy, hyperthermia and supporting measures. Springer; 2011. p.25-37.

Lalonde R, Strazielle C. Brain regions and genes affecting postural control. Progress in Neurobiology 2007;81(1):45-60.

Shiotsuki H, Yoshimi K, Shimo Y, Funayama M, Funayama M, Takamatsu Y et al. A rotarod test for evaluation of motor skill learning. J Neurosci Methods 2010;189(2):180-5.

Navarro A, Gomez C, Lopez-Cepero JM, Boveris A. Beneficial effects of moderate exercise on mice aging: survival, behavior, oxidative stress, and mitochondrial electron transfer. Am J Physiol Regul Integr Comp Physiol 2004;286(3):R505-R11.

Chabner BA, Londo DL. Cancer chemotherapy and biotherapy: principles and practice 4ª ed. Illinois: Wolters Kluwer; 2005.

Radak Z, Toldy A, Szabo Z, Siamilis S, Nyakas C, Silye G et al. The effects of training and detraining on memory, neurotrophins and oxidative stress markers in rat brain. Neurochem Int 2006;49(4):387-92.

Heo YM, Shin MS, Lee JM, Kim CJ, Baek SB, Kim KH, Baek SS. Treadmill exercise ameliorates short-term memory disturbance in scopolamine-induced amnesia rats. Int Neurourol J 2014;18(1):16-22.

National Heart, Lung and Blood Institute. what is thrombocytopenia? [cited Aug 12]. Disponível:

Valenti M, Porzio G, Aielli F, Verna L, Cannita K, Manno R et al. Physical exercise and quality of life in breast cancer survivors. Int J Medical Sci 2008;5(1):24.

Stephenson LE, Bebb DG, Reimer RA, Culos-Reed SN. Physical activity and diet behaviour in colorectal cancer patients receiving chemotherapy: associations with quality of life. BMC Gastroenterol 2009;9(1):60.



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